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Determination Of Caffeine In Coffee High Performance Liquid Chromatography

Sep 3, 2021 | SCIENCE | 0 comments

Sep 3, 2021 | SCIENCE | 0 comments

Determination Of Caffeine In Coffee High Performance Liquid Chromatography

Determination Of Caffeine In Coffee High Performance Liquid Chromatography

1-write an experimental part of HPLC experiment following “4.” format that in the attachment
we did the procedure on page 348, please do not forget those info aboutthe instrument we used.
-Hewlitt-Packard HPLC HP Series 1100
-Column: C18 reverse phase, 5 micrometer particle size, 50 mm x 4.6 mm
-Detector: variable wavelength (set to 254 nm)
-Integrator: HP 6890
-Our analysis uses the (aqueous) phosphate buffer instead of the water, so the mobile phase is phosphate buffer/methanol 80/20.
2-discuss ALL the result of HPLC experiment and make sure to find these two things:
a) The concentration (mg/L) of caffeine in the original beverage that you calculated from the chromatogram.
b) compare results obtained above with those on beverage container in both concentration (mg/L) AND total caffeine content (mg/container)]
You have to use very easy and simple english words and perfect grammar

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Attachments
20190416220120procedure (5 MB)
201904162201311045_001 (66 kB)
201904162201321046_001 (21 kB)

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